Functional prediction of miR-3144-5p in human cardiac myocytes based on transcriptome sequencing and bioinformatics.

ABSTRACT:

Background

RAN guanine nucleotide release factor (RANGRF) encoding protein MOG1 plays an important role in cardiac arrhythmia, so we intended to investigate the regulatory miRNA of RANGRF and explore its potential regulatory mechanism in arrhythmogenesis.

Methods

Based on bioinformatic analysis, miR-3144-5p was predicted to be a regulatory miRNA of RANGRF, which were then validated through a dual-luciferase reporter plasmid assay. Subsequently, the expression level of miR-3144-5p in human cardiac myocytes (HCMs) was detected, followed by cell transfection with miR-3144-5p mimics. Transcriptome sequencing was then performed in HCMs with or without transfection. The sequencing results were subjected to bioinformatic analyses, including differentially expressed gene (DEG) analy

SUBMITTER: Ruan L 

PROVIDER: S-EPMC5556203 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature