Stathmin is overexpressed and regulated by mutant p53 in oral squamous cell carcinoma.

ABSTRACT:

Background

The aim of this study was to investigate the oncogenic function and regulatory mechanism of stathmin in oral squamous cell carcinoma (OSCC).

Methods

Two-dimensional electrophoresis and liquid chromatography-tandem mass chromatography were applied to screen differentiated proteins during carcinogenesis in OSCC. Cell Counting Kit-8 (CCK-8) assays, colony formation, migration, flow cytometry, immunofluorescence and a xenograft model were used to detect the function of stathmin. The correlation between stathmin and p53 expression was analyzed using immunohistochemistry. Mutant/wild type p53 plasmids and small interfering RNA were used to examine the regulation of stathmin. Chromatin immunoprecipitation assays and luciferase assays were performed to detect the transcri

SUBMITTER: Ma HL 

PROVIDER: S-EPMC5556353 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature