Responses of Synechocystis sp. PCC 6803 to heterologous biosynthetic pathways.

ABSTRACT:

Background

There are an increasing number of studies regarding genetic manipulation of cyanobacteria to produce commercially interesting compounds. The majority of these works study the expression and optimization of a selected heterologous pathway, largely ignoring the wholeness and complexity of cellular metabolism. Regulation and response mechanisms are largely unknown, and even the metabolic pathways themselves are not fully elucidated. This poses a clear limitation in exploiting the rich biosynthetic potential of cyanobacteria.

Results

In this work, we focused on the production of two different compounds, the cyanogenic glucoside dhurrin and the diterpenoid 13R-manoyl oxide in Synechocystis PCC 6803. We used genome-scale metabolic modelling to study fluxes in individual

SUBMITTER: Vavitsas K 

PROVIDER: S-EPMC5556357 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature