Age-associated mRNA expression changes in bovine endometrial cells in vitro.

ABSTRACT:

Background

Endometrial cells secrete various cytokines and the dysfunction of endometrial cells may directly lead to infertility. Interferon tau (IFNT) secreted by trophoblast cells, a well-known pregnancy recognition signal in ruminants, acts on the uterus to prepare for pregnancy. Aging causes cellular and organ dysfunction, and advanced maternal age is associated with reduced fertility. However, few studies have investigated age-dependent changes in the uterus.

Methods

Using next generation sequencing and real-time PCR, we examined mRNA expression in bovine endometrial cells in vitro obtained from young (mean 45.2 months) and aged (mean 173.5 months) animals and the effects of IFNT depending on the age.

Results

We showed that inflammation-related (predicted molecul

SUBMITTER: Tanikawa N 

PROVIDER: S-EPMC5556672 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature