Fluorescent carbon nanoparticle-based lateral flow biosensor for ultrasensitive detection of DNA.

ABSTRACT: We report a fluorescent carbon nanoparticle (FCN)-based lateral flow biosensor for ultrasensitive detection of DNA. Fluorescent carbon nanoparticle with a diameter of around 15nm was used as a tag to label a detection DNA probe, which was complementary with the part of target DNA. A capture DNA probe was immobilized on the test zone of the lateral flow biosensor. Sandwich-type hybridization reactions among the FCN-labeled DNA probe, target DNA and capture DNA probe were performed on the lateral flow biosensor. In the presence of target DNA, FCNs were captured on the test zone of the biosensor and the fluorescent intensity of the captured FCNs was measured with a portable fluorescent reader. After systematic optimizations of experimental parameters (the components of running buffers, the co

SUBMITTER: Takalkar S 

PROVIDER: S-EPMC5556692 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature