Acute Elevated Glucose Promotes Abnormal Action Potential-Induced Ca²⁺ Transients in Cultured Skeletal Muscle Fibers.

ABSTRACT: A common comorbidity of diabetes is skeletal muscle dysfunction, which leads to compromised physical function. Previous studies of diabetes in skeletal muscle have shown alterations in excitation-contraction coupling (ECC)-the sequential link between action potentials (AP), intracellular Ca²⁺ release, and the contractile machinery. Yet, little is known about the impact of acute elevated glucose on the temporal properties of AP-induced Ca²⁺ transients and ionic underlying mechanisms that lead to muscle dysfunction. Here, we used high-speed confocal Ca²⁺ imaging to investigate the temporal properties of AP-induced Ca²⁺ transients, an intermediate step of ECC, using an acute in cellulo model of uncontrolled hyperglycemia (25 mM, 48 h.). Control and

SUBMITTER: Hernandez-Ochoa EO 

PROVIDER: S-EPMC5557004 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature