New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica.

ABSTRACT:

Background

The oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At present, several promoters with different strengths have been developed based either on the constitutive pTEF promoter or on oleic acid inducible promoters such as pPOX2 and pLIP2. Although these promoters are highly efficient, there is still a lack of versatile inducible promoters for gene expression in Y. lipolytica.

Results

We have isolated and characterized the promoter of the EYK1 gene coding for an erythrulose kinase. pEYK1 induction was found to be impaired in media supplemented with glucose and glycerol, while the presence of erythritol and erythrulose strongly increased the promoter induction level. Promoter characteriza

SUBMITTER: Trassaert M 

PROVIDER: S-EPMC5557077 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature