Transcriptomic profile of tobacco in response to Tomato zonate spot orthotospovirus infection.

ABSTRACT:

Background

Tomato zonate spot virus (TZSV), a dominant species of thrips-transmitted orthotospoviruses in Yunnan and Guangxi provinces in China, causes significant loss of yield in lots of crops and is a major threat to incomes of rural families. However, the detailed molecular mechanism of crop disease caused by TZSV remains obscure.

Methods

Next-generation sequencing (NGS)-based transcriptome analysis (RNA-seq) was performed to investigate and compare the gene expression changes in systemic leaves of tobacco upon infection with TZSV and mock-inoculated plants as a control.

Results

De novo assembly and analysis of tobacco transcriptome data by RNA-Seq identified 135,395 unigenes. 2102 differentially expressed genes (DEGs) were obtained in tobacco with TZSV infection,

SUBMITTER: Huang C 

PROVIDER: S-EPMC5557316 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature