Mechanism of Substrate Translocation in an Alternating Access Transporter.

ABSTRACT: Transporters shuttle molecules across cell membranes by alternating among distinct conformational states. Fundamental questions remain about how transporters transition between states and how such structural rearrangements regulate substrate translocation. Here, we capture the translocation process by crystallography and unguided molecular dynamics simulations, providing an atomic-level description of alternating access transport. Simulations of a SWEET-family transporter initiated from an outward-open, glucose-bound structure reported here spontaneously adopt occluded and inward-open conformations. Strikingly, these conformations match crystal structures, including our inward-open structure. Mutagenesis experiments further validate simulation predictions. Our results reveal that state tra

SUBMITTER: Latorraca NR 

PROVIDER: S-EPMC5557413 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature