Protein deubiquitinase USP7 is required for osteogenic differentiation of human adipose-derived stem cells.

ABSTRACT:

Background

Human adipose-derived stem cells (hASCs) are multipotent progenitor cells with self-renewal capabilities and multilineage differentiation potential, including osteogenesis. Although protein deubiquitinases have been linked to stem cell fate determination, whether protein deubiquitination contributes to lineage commitment during osteogenic differentiation of hASCs remains to be investigated. The objective of this study was to evaluate the effects of the ubiquitin specific protease 7 (USP7) on osteogenic differentiation of hASCs.

Methods

An osteocalcin promoter driven luciferase reporter system was established to initially discover the potential association between USP7 and hASC osteogenesis. To further characterize the function of USP7 in osteogenic differentiation

SUBMITTER: Tang Y 

PROVIDER: S-EPMC5557518 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature