Next generation sequencing reveals the antibiotic resistant variants in the genome of Pseudomonas aeruginosa.

ABSTRACT: Rapid progress in next generation sequencing and allied computational tools have aided in identification of single nucleotide variants in genomes of several organisms. In the present study, we have investigated single nucleotide polymorphism (SNP) in ten multi-antibiotic resistant Pseudomonas aeruginosa clinical isolates. All the draft genomes were submitted to Rapid Annotations using Subsystems Technology (RAST) web server and the predicted protein sequences were used for comparison. Non-synonymous single nucleotide polymorphism (nsSNP) found in the clinical isolates compared to the reference genome (PAO1), and the comparison of nsSNPs between antibiotic resistant and susceptible clinical isolates revealed insights into the genome variation. These nsSNPs identified in the multi-drug resis

SUBMITTER: Ramanathan B 

PROVIDER: S-EPMC5557631 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature