Computational identification of Y-linked markers and genes in the grass carp genome by using a pool-and-sequence method.

ABSTRACT: The molecular analysis of sex in vertebrates is important, as it has the potential to provide vital information for theoretical and applied research alike. Teleost fish are the ancient vertebrates that present a broad sex chromosome system but lack differentiated sex chromosomes in most species. Hence understanding the sex in fish would not only illuminate the sex determination evolution in vertebrates but also shed light on fish farming. In the present study, we used grass carp as a teleost fish model, studied the Y chromosome by using a pool-and-sequence strategy in combination with fragment-ratio method. In total, we identified five Y-linked scaffolds (totaling 347 Kb) and six Y-specific sequences that could be used as sex-specific markers, demonstrating the suitability of NGS-based re-

SUBMITTER: Zhang A 

PROVIDER: S-EPMC5557828 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature