Expression of Interleukin-26 is upregulated in inflammatory bowel disease.

ABSTRACT:

Aim

To investigate interleukin (IL)-26 expression in the inflamed mucosa of patients with inflammatory bowel disease (IBD) and the function of IL-26.

Methods

Human colonic subepithelial myofibroblasts (SEMFs) were isolated from colon tissue surgically resected. The expression of IL-26 protein and its receptor complex was analyzed by immunohistochemistry. The gene expression induced by IL-26 was evaluated by real-time polymerase chain reaction. Intracellular signaling pathways were evaluated by immunoblotting and specific small interfering (si) RNA transfection.

Results

The mRNA and protein expression of IL-26 were significantly enhanced in the inflamed mucosa of patients with IBD. IL-26 receptor complex was expressed in colonic SEMFs in vivo and in vitro

SUBMITTER: Fujii M 

PROVIDER: S-EPMC5558115 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature