Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification.

ABSTRACT:

Background

Canine distemper, caused by Canine distemper virus (CDV), is a highly contagious and fatal systemic disease in free-living and captive carnivores worldwide. Recombinase polymerase amplification (RPA), as an isothermal gene amplification technique, has been explored for the molecular detection of diverse pathogens.

Methods

A real-time reverse transcription RPA (RT-RPA) assay for the detection of canine distemper virus (CDV) using primers and exo probe targeting the CDV nucleocapsid protein gene was developed. A series of other viruses were tested by the RT-RPA.Thirty-two field samples were further tested by RT-RPA, and the resuts were compared with those obtained by the real-time RT-PCR.

Results

The RT-RPA assay was performed successfully at 40 °C, and the r

SUBMITTER: Wang J 

PROVIDER: S-EPMC5558738 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature