A novel RNA polymerase-binding protein that interacts with a sigma-factor docking site.

ABSTRACT: Sporulation in Bacillus subtilis is governed by a cascade of alternative RNA polymerase sigma factors. We previously identified a small protein Fin that is produced under the control of the sporulation sigma factor σ^F to create a negative feedback loop that inhibits σ^F -directed gene transcription. Cells deleted for fin are defective for spore formation and exhibit increased levels of σ^F -directed gene transcription. Based on pull-down experiments, chemical crosslinking, bacterial two-hybrid experiments and nuclear magnetic resonance chemical shift analysis, we now report that Fin binds to RNA polymerase and specifically to the coiled-coil region of the β' subunit. The coiled-coil is a docking site for sigma factors on RNA polymerase, and evidence is presen

SUBMITTER: Wang Erickson AF 

PROVIDER: S-EPMC5558796 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature