Temperature-responsive miRNAs in Drosophila orchestrate adaptation to different ambient temperatures.

ABSTRACT: The majority of Drosophila genes are expressed in a temperature-dependent manner, but the way in which small RNAs may contribute to this effect is completely unknown as we currently lack an idea of how small RNA transcriptomes change as a function of temperature. Applying high-throughput sequencing techniques complemented by quantitative real-time PCR experiments, we demonstrate that altered ambient temperature induces drastic but reversible changes in sequence composition and total abundance of both miRNA and piRNA populations. Further, mRNA sequencing reveals that the expression of miRNAs and their predicted target transcripts correlates inversely, suggesting that temperature-responsive miRNAs drive adaptation to different ambient temperatures on the transcriptome level. Finally,

SUBMITTER: Fast I 

PROVIDER: S-EPMC5558905 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature