Deacetylase activity of histone deacetylase 3 is required for productive VDJ recombination and B-cell development.

ABSTRACT: Histone deacetylase 3 (HDAC3) is the catalytic component of NCoR/SMRT corepressor complexes that mediate the actions of transcription factors implicated in the regulation of B-cell development and function. We crossed Hdac3 conditional knockout mice with Mb1-Cre knockin animals to delete Hdac3 in early progenitor B cells. The spleens of Hdac3^F/-Mb1-Cre^+/- mice were virtually devoid of mature B cells, and B220⁺CD43⁺ B-cell progenitors accumulated within the bone marrow. Quantitative deep sequencing of the Ig heavy chain locus from B220⁺CD43⁺ populations identified a defect in V_HDJ_H recombination with a severe reduction in productive rearrange

SUBMITTER: Stengel KR 

PROVIDER: S-EPMC5559004 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature