Effects of Detergent Micelles on Lipid Binding to Proteins in Electrospray Ionization Mass Spectrometry.

ABSTRACT: A wide variety of biological processes rely upon interactions between proteins and lipids, ranging from molecular transport to the organization of the cell membrane. It was recently established that electrospray ionization mass spectrometry (ESI-MS) is capable of capturing transient interactions between membrane proteins and their lipid environment, and a detailed understanding of the underlying processes is therefore of high importance. Here, we apply ESI-MS to investigate the factors that govern complex formation in solution and gas phases by comparing nonselective lipid binding with soluble and membrane proteins. We find that exogenously added lipids did not bind to soluble proteins, suggesting that lipids have a low propensity to form electrospray ionization adducts. The presence of de

SUBMITTER: Landreh M 

PROVIDER: S-EPMC5559180 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature