The [PSI ⁺] yeast prion does not wildly affect proteome composition whereas selective pressure exerted on [PSI ⁺] cells can promote aneuploidy.

ABSTRACT: The yeast Sup35 protein is a subunit of the translation termination factor, and its conversion to the [PSI ⁺] prion state leads to more translational read-through. Although extensive studies have been done on [PSI ⁺], changes at the proteomic level have not been performed exhaustively. We therefore used a SILAC-based quantitative mass spectrometry approach and identified 4187 proteins from both [psi ^-] and [PSI ⁺] strains. Surprisingly, there was very little difference between the two proteomes under standard growth conditions. We found however that several [PSI ⁺] strains harbored an additional chromosome, such as chromosome I. Albeit, we found no evidence to support that [PSI ⁺] induces chromosomal instability (CIN). Inste

SUBMITTER: Chan PHW 

PROVIDER: S-EPMC5559586 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature