Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration.

ABSTRACT: Zebrafish have the capacity to regenerate lost tissues and organs. Amputation of the caudal fin results in a rapid, transient increase in H₂O₂ levels emanating from the wound margin, which is essential for regeneration, because quenching of reactive oxygen species blocks regeneration. Protein-tyrosine phosphatases (PTPs) have a central role in cell signalling and are susceptible to oxidation, which results in transient inactivation of their catalytic activity. We hypothesized that PTPs may become oxidized in response to amputation of the caudal fin. Using the oxidized PTP-specific (ox-PTP) antibody and liquid chromatography-mass spectrometry, we identified 33 PTPs in adult zebrafish fin clips of the total of 44 PTPs that can theoretically be detected based on sequence

SUBMITTER: Wu W 

PROVIDER: S-EPMC5559610 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature