Defining the DNA Binding Site Recognized by the Fission Yeast Zn₂Cys₆ Transcription Factor Pho7 and Its Role in Phosphate Homeostasis.

ABSTRACT: Fission yeast phosphate homeostasis entails transcriptional induction of genes encoding phosphate-mobilizing proteins under conditions of phosphate starvation. Transcription factor Pho7, a member of the Zn₂Cys₆ family of fungal transcription regulators, is the central player in the starvation response. The DNA binding sites in the promoters of phosphate-responsive genes have not been defined, nor have any structure-function relationships been established for the Pho7 protein. Here we narrow this knowledge gap by (i) delineating an autonomous DNA-binding domain (DBD) within Pho7 that includes the Zn₂Cys₆ module, (ii) deploying recombinant Pho7 DBD in DNase I footprinting and electrophoretic mobility shift assays (EMSAs) to map the Pho7 recognition

SUBMITTER: Schwer B 

PROVIDER: S-EPMC5559640 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature