Multiplication of Ribosomal P-Stalk Proteins Contributes to the Fidelity of Translation.

ABSTRACT: The P-stalk represents a vital element within the ribosomal GTPase-associated center, which represents a landing platform for translational GTPases. The eukaryotic P-stalk exists as a uL10-(P1-P2)2 pentameric complex, which contains five identical C-terminal domains, one within each protein, and the presence of only one such element is sufficient to stimulate factor-dependent GTP hydrolysis in vitro and to sustain cell viability. The functional contribution of the P-stalk to the performance of the translational machinery in vivo, especially the role of P-protein multiplication, has never been explored. Here, we show that ribosomes depleted of P1/P2 proteins exhibit reduced translation fidelity at elongation and termination steps. The elevated rate of the decoding error is inversely correla

SUBMITTER: Wawiorka L 

PROVIDER: S-EPMC5559677 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature