OmpA protein sequence-based typing and virulence-associated gene profiles of Pasteurella multocida isolates associated with bovine haemorrhagic septicaemia and porcine pneumonic pasteurellosis in Thailand.

ABSTRACT:

Background

Pasteurella multocida is a Gram-negative bacterium that causes economically significant infections of a broad range of animal species. Pneumonic and septicaemic pasteurellosis caused by this bacterium remain important problems in pigs, cattle, and water buffaloes in Thailand. The aim of this study was to characterise the virulence-associated gene profiles and to develop an OmpA molecular typing scheme for classifying 191 bovine and porcine isolates of P. multocida collected between 1989 and 2012 in Thailand using polymerase chain reactions (PCRs), nucleotide sequencing, and sequence and structural bioinformatics analyses.

Results

PCR screening successfully characterised the profiles of 25 virulence-associated genes in all isolates. The gene profiles separated thes

SUBMITTER: E-Kobon T 

PROVIDER: S-EPMC5559837 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature