Wnt signalling controls the response to mechanical loading during zebrafish joint development.

ABSTRACT: Joint morphogenesis requires mechanical activity during development. Loss of mechanical strain causes abnormal joint development, which can impact long-term joint health. Although cell orientation and proliferation are known to shape the joint, dynamic imaging of developing joints in vivo has not been possible in other species. Using genetic labelling techniques in zebrafish we were able, for the first time, to dynamically track cell behaviours in intact moving joints. We identify that proliferation and migration, which contribute to joint morphogenesis, are mechanically controlled and are significantly reduced in immobilised larvae. By comparison with strain maps of the developing skeleton, we identify canonical Wnt signalling as a candidate for transducing mechanical forces into j

SUBMITTER: Brunt LH 

PROVIDER: S-EPMC5560048 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature