Freeze-thaw decellularization of the trabecular meshwork in an ex vivo eye perfusion model.

ABSTRACT:

Objective

The trabecular meshwork (TM) is the primary substrate of outflow resistance in glaucomatous eyes. Repopulating diseased TM with fresh, functional TM cells might be a viable therapeutic approach. Decellularized TM scaffolds have previously been produced by ablating cells with suicide gene therapy or saponin, which risks incomplete cell removal or dissolution of the extracellular matrix, respectively. We hypothesized that improved trabecular meshwork cell ablation would result from freeze-thaw cycles compared to chemical treatment.

Materials and methods

We obtained 24 porcine eyes from a local abattoir, dissected and mounted them in an anterior segment perfusion within two hours of sacrifice. Intraocular pressure (IOP) was recorded continuously by a pressure transduc

SUBMITTER: Dang Y 

PROVIDER: S-EPMC5560227 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature