Characterization of amyloid-β precursor protein intracellular domain-associated transcriptional complexes in SH-SY5Y neurocytes.

ABSTRACT:

Objective

Alzheimer's disease (AD) is one of the major disorders worldwide. Recent research suggests that the amyloid-β precursor protein intracellular domain (AICD) is a potential contributor to AD development and progression. The small AICD is rapidly degraded after processing from the full-length protein. The present study aimed to apply a highly efficient biotinylation approach in vitro to study AICD-associated complexes in neurocytes.

Methods

By co-expressing Escherichia coli biotin ligase with biotinyl-tagged AICD in the SH-SY5Y neuronal cell line, the effects of AICD overexpression on cell proliferation and apoptosis were analyzed. Besides, AICD-associated nuclear transcriptional complexes were purified and then examined by mass spectrometry.

Results

Our data s

SUBMITTER: Yang W 

PROVIDER: S-EPMC5560331 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature