Assays for precise quantification of total (including short) and elongated HIV-1 transcripts.

ABSTRACT: Despite intensive study, it is unclear which mechanisms are responsible for latent HIV infection in vivo. One potential mechanism is inhibition of HIV transcriptional elongation, which results in short abortive transcripts containing the trans-activation response (TAR) region. Because the relative levels of total (including short) and processive transcripts provide measures of HIV transcriptional initiation and elongation, there is a compelling need for techniques that accurately measure both. Nonetheless, prior assays for total transcripts have been semi-quantitative and have seen limited application to patient samples. This manuscript reports the validation of quantitative reverse transcription (RT) droplet digital PCR assays for measurement of total (TAR) and processive (R-U5/gag) HIV t

SUBMITTER: Kaiser P 

PROVIDER: S-EPMC5560404 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature