NKG2D ligand expression in Crohn's disease and NKG2D-dependent stimulation of CD8⁺ T cell migration.

ABSTRACT: Interaction between the activating NKG2D receptor on lymphocytes and its ligands MICA, MICB, and ULBP1-6 modulate T and NK cell activity and may contribute to the pathogenesis of Crohn's disease (CD). NKG2D ligands are generally not expressed on the cell surface of normal, non-stressed cells, but expression of MICA and MICB in CD intestine has been reported. In this exploratory study, we further characterize the expression of NKG2D and its ligands, including the less well-described ULBP4-6, in CD, and test if NKG2D ligand interactions are involved in the migration of activated T cells into the affected mucosal compartments. Intestinal tissue from CD patients and healthy controls were analyzed by flow cytometry, mass cytometry, and immunohistochemistry for expression of NKG2D and ligands, a

SUBMITTER: Vadstrup K 

PROVIDER: S-EPMC5560431 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature