Pressure in isochoric systems containing aqueous solutions at subzero Centigrade temperatures.

ABSTRACT:

Objective

Preservation of biological materials at subzero Centigrade temperatures, cryopreservation, is important for the field of tissue engineering and organ transplantation. Our group is studying the use of isochoric (constant volume) systems of aqueous solution for cryopreservation. Previous studies measured the pressure-temperature relations in aqueous isochoric systems in the temperature range from 0°C to - 20°C. The goal of this study is to expand the pressure-temperature measurement beyond the range reported in previous publications.

Materials and methods

To expand the pressure-temperature measurements beyond the previous range, we have developed a new isochoric device capable of withstanding liquid nitrogen temperatures and pressures of up to 413 MPa. The device is

SUBMITTER: Ukpai G 

PROVIDER: S-EPMC5560655 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature