MALDI-TOF MS identification of Anopheles gambiae Giles blood meal crushed on Whatman filter papers.

ABSTRACT:

Background

Identification of the source of mosquito blood meals is an important component for disease control and surveillance. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an effective tool for mosquito blood meal identification, using the abdomens of freshly engorged mosquitoes. In the field, mosquito abdomens are crushed on Whatman filter papers to determine the host feeding patterns by identifying the origin of their blood meals. The aim of this study was to test whether crushing engorged mosquito abdomens on Whatman filter papers was compatible with MALDI-TOF MS for mosquito blood meal identification. Both laboratory reared and field collected mosquitoes were tested.

Material and methods

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SUBMITTER: Niare S 

PROVIDER: S-EPMC5560667 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature