Cis- and Trans-regulatory Effects on Gene Expression in a Natural Population of Drosophila melanogaster.

ABSTRACT: Cis- and trans-regulatory mutations are important contributors to transcriptome evolution. Quantifying their relative contributions to intraspecific variation in gene expression is essential for understanding the population genetic processes that underlie evolutionary changes in gene expression. Here, we have examined this issue by quantifying genome-wide, allele-specific expression (ASE) variation using a crossing scheme that produces F₁ hybrids between 18 different Drosophila melanogaster strains sampled from the Drosophila Genetic Reference Panel and a reference strain from another population. Head and body samples from F₁ adult females were subjected to RNA sequencing and the subsequent ASE quantification. Cis- and trans-r

SUBMITTER: Osada N 

PROVIDER: S-EPMC5560811 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature