Networks Underpinning Symbiosis Revealed Through Cross-Species eQTL Mapping.

ABSTRACT: Organisms engage in extensive cross-species molecular dialog, yet the underlying molecular actors are known for only a few interactions. Many techniques have been designed to uncover genes involved in signaling between organisms. Typically, these focus on only one of the partners. We developed an expression quantitative trait locus (eQTL) mapping-based approach to identify cause-and-effect relationships between genes from two partners engaged in an interspecific interaction. We demonstrated the approach by assaying expression of 98 isogenic plants (Medicago truncatula), each inoculated with a genetically distinct line of the diploid parasitic nematode Meloidogyne hapla With this design, systematic differences in gene expression across host plants could be mapped to genetic po

SUBMITTER: Guo Y 

PROVIDER: S-EPMC5560814 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature