A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity.

ABSTRACT: Due to their small dimensions, electrophysiology on thin and intricate axonal branches in support of understanding their role in normal and diseased brain function poses experimental challenges. To reduce experimental complexity, we coupled microelectrode arrays (MEAs) to bi-level microchannel devices for the long-term in vitro tracking of axonal morphology and activity with high spatiotemporal resolution. Our model allowed the long-term multisite recording from pure axonal branches in a microscopy-compatible environment. Compartmentalizing the network structure into interconnected subpopulations simplified access to the locations of interest. Electrophysiological data over 95 days in vitro (DIV) showed an age-dependent increase of axonal conduction velocity, which was positively correlate

SUBMITTER: Habibey R 

PROVIDER: S-EPMC5561146 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature