Mechanism of transformation in Mycobacteria using a novel shockwave assisted technique driven by in-situ generated oxyhydrogen.

ABSTRACT: We present a novel method for shockwave-assisted bacterial transformation using a miniature oxyhydrogen detonation-driven shock tube. We have obtained transformation efficiencies of about 1.28 × 10⁶, 1.7 × 10⁶, 5 × 10⁶, 1 × 10⁵, 1 × 10⁵ and 2 × 10⁵ transformants/µg of DNA for Escherichia coli, Salmonella Typhimurum, Pseudomonas aeruginosa, Mycobacterium smegmatis, Mycobacterium tuberculosis (Mtb) and Helicobacter pylori respectively using this method which are significantly higher than those obtained using conventional methods. Mtb is the most difficult bacteria to be transformed and hence their genetic modification is hampered due to their poor transformation efficiency. Experimental results show that longer steady time dur

SUBMITTER: Datey A 

PROVIDER: S-EPMC5561244 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature