MiR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1.

ABSTRACT:

Background

The aberrant expression of microRNA-140-5p (miR-140-5p) has been described in gastric cancer (GC). However, the role of miR-140-5p in GC remains unclear. In this study, the prognostic relevance of miR-140-5p in GC was investigated and YES1 was identified as a novel target of miR-140-5p in regulating tumor progression.

Methods

miR-140-5p level was determined in 20 paired frozen specimens through quantitative real-time PCR, and analyzed in tissue microarrays through in situ hybridization. The target of miR-140-5p was verified through a dual luciferase reporter assay, and the effects of miR-140-5p on phenotypic changes in GC cells were investigated in vitro and in vivo.

Results

Compared with that in adjacent normal tissues, miR-140-5p expression decreased in c

SUBMITTER: Fang Z 

PROVIDER: S-EPMC5561618 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature