Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica.

ABSTRACT:

Background

An inducible promoter for recombinant protein expression provides substantial benefits because under induction conditions cellular energy and metabolic capability can be directed into protein synthesis. The most widely used inducible promoter for diatoms is for nitrate reductase, however, nitrogen metabolism is tied into diverse aspects of cellular function, and the induction response is not necessarily robust. Silicon limitation offers a means to eliminate energy and metabolic flux into cell division processes, with little other detrimental effect on cellular function, and a protein expression system that works under those conditions could be advantageous.

Results

In this study, we evaluate a number of promoters for recombinant protein expression induced by silic

SUBMITTER: Shrestha RP 

PROVIDER: S-EPMC5561644 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature