Direct detection of Helicobacter pylori in biopsy specimens using a high-throughput multiple genetic detection system.

ABSTRACT:

Aim

We evaluated the direct high-throughput multiple genetic detection system (dHMGS) for Helicobacter pylori in gastric biopsies.

Materials & methods

One hundred and thirty-three specimens were concurrently analyzed by dHMGS, rapid urease test, culture and sequencing.

Results

dHMGS was highly sensitive and specific for H. pylori identification compared with culture and rapid urease test. The correlation coefficient of the quantitative standard curve was R² = 0.983. A significant difference in the relative H. pylori DNA abundance was found in different gastroduodenal diseases. Concordance rates between dHMGS and sequencing for resistance mutations were 97.1, 100.0, 85.3 and 97.1%, respectively. Finally, dHMGS could efficiently distinguish mixed infection in

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5562010 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature