Epac1-deficient mice have bleeding phenotype and thrombocytes with decreased GPIbβ expression.

ABSTRACT: Epac1 (Exchange protein directly activated by cAMP 1) limits fluid loss from the circulation by tightening the endothelial barrier. We show here that Epac1^-/- mice, but not Epac2^-/- mice, have prolonged bleeding time, suggesting that Epac1 may limit fluid loss also by restraining bleeding. The Epac1^-/- mice had deficient in vitro secondary hemostasis. Quantitative comprehensive proteomics analysis revealed that Epac1^-/- mouse platelets (thrombocytes) had unbalanced expression of key components of the glycoprotein Ib-IX-V (GPIb-IX-V) complex, with decrease of GP1bβ and no change of GP1bα. This complex is critical for platelet adhesion under arterial shear conditions. Furthermore, Epac1^-/- mice have reduced levels of plasma coagulation f

SUBMITTER: Nygaard G 

PROVIDER: S-EPMC5562764 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature