Overcoming the plasticity of plant specialized metabolism for selective diterpene production in yeast.

ABSTRACT: Plants synthesize numerous specialized metabolites (also termed natural products) to mediate dynamic interactions with their surroundings. The complexity of plant specialized metabolism is the result of an inherent biosynthetic plasticity rooted in the substrate and product promiscuity of the enzymes involved. The pathway of carnosic acid-related diterpenes in rosemary and sage involves promiscuous cytochrome P450s whose combined activity results in a multitude of structurally related compounds. Some of these minor products, such as pisiferic acid and salviol, have established bioactivity, but their limited availability prevents further evaluation. Reconstructing carnosic acid biosynthesis in yeast achieved significant titers of the main compound but could not specifically yield the minor

SUBMITTER: Ignea C 

PROVIDER: S-EPMC5562805 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature