Directed evolution of specific receptor-ligand pairs for use in the creation of gene switches.

ABSTRACT: Despite their versatility and power in controlling gene regulation in nature, nuclear hormone receptors (NHRs) have largely eluded utility in heterologous gene regulation applications such as gene therapy and metabolic engineering. The main reason for this void is the pleiotropic interference of the receptor-ligand combination with regulatory networks in the host organism. In recent years, numerous strategies have been developed to engineer ligand-receptor pairs that do not cross-interact with host regulatory pathways. However, these strategies have either met with limited success or cannot be readily extended to other ligand-receptor pairs. Here, we present a simple, effective, and readily generalizable strategy for reengineering NHRs to respond specifically to a selected synthetic ligand

SUBMITTER: Chockalingam K 

PROVIDER: S-EPMC556283 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature