Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system.

ABSTRACT: The enteric nervous system (ENS) of the gastrointestinal tract controls many diverse functions, including motility and epithelial permeability. Perturbations in ENS development or function are common, yet there is no human model for studying ENS-intestinal biology and disease. We used a tissue-engineering approach with embryonic and induced pluripotent stem cells (PSCs) to generate human intestinal tissue containing a functional ENS. We recapitulated normal intestinal ENS development by combining human-PSC-derived neural crest cells (NCCs) and developing human intestinal organoids (HIOs). NCCs recombined with HIOs in vitro migrated into the mesenchyme, differentiated into neurons and glial cells and showed neuronal activity, as measured by rhythmic waves of calcium transients. ENS-containi

SUBMITTER: Workman MJ 

PROVIDER: S-EPMC5562951 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature