Impact of blood collection and processing on peripheral blood gene expression profiling in type 1 diabetes.

ABSTRACT:

Background

The natural history of type 1 diabetes (T1D) is challenging to investigate, especially as pre-diabetic individuals are difficult to identify. Numerous T1D consortia have been established to collect whole blood for gene expression analysis from individuals with or at risk to develop T1D. However, with no universally accepted protocol for their collection, differences in sample processing may lead to variances in the results. Here, we examined whether the choice of blood collection tube and RNA extraction kit leads to differences in the expression of genes that are changed during the progression of T1D, and if these differences could be minimized by measuring gene expression directly from the lysate of whole blood.

Results

Microarray analysis showed that the express

SUBMITTER: Yip L 

PROVIDER: S-EPMC5563008 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature