Acute Penicillium marneffei infection stimulates host M1/M2a macrophages polarization in BALB/C mice.

ABSTRACT:

Background

Penicillium marneffei (P. marneffei) is a thermally dimorphic fungus pathogen that causes fatal infection. Alveolar macrophages are innate immune cells that have critical roles in protection against pulmonary fungal pathogens and the macrophage polarization state has the potential to be a deciding factor in disease progression or resolution. The aim of this study was to investigate mouse alveolar macrophage polarization states during P. marneffei infection.

Results

We used enzyme-linked immunosorbent (ELISA) assays, quantitative real-time PCR (qRT-PCR), and Griess, arginase activity to evaluate the phenotypic markers of alveolar macrophages from BALB/C mice infected with P. marneffei. We then treated alveolar macrophages from infected mice with P. marneffei cytopl

SUBMITTER: Dai X 

PROVIDER: S-EPMC5563047 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature