A cost-effective approach to produce ¹⁵N-labelled amino acids employing Chlamydomonas reinhardtii CC503.

ABSTRACT:

Background

The use of enriched stable isotopes is of outstanding importance in chemical metrology as it allows the application of isotope dilution mass spectrometry (IDMS). Primary methods based on IDMS ensure the quality of the analytical measurements and traceability of the results to the international system of units. However, the synthesis of isotopically labelled molecules from enriched stable isotopes is an expensive and a difficult task. Either chemical and biochemical methods to produce labelled molecules have been proposed, but so far, few cost-effective methods have been described.

Results

The aim of this study was to use the microalgae Chlamydomonas reinhardtii to produce, at laboratory scale, ¹⁵N-labelled amino acids with a high isotopic enrichment. To

SUBMITTER: Nicolas Carcelen J 

PROVIDER: S-EPMC5563056 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature