Can the propensity of protein crystallization be increased by using systematic screening with metals?

ABSTRACT: Protein crystallization is one of the major bottlenecks in protein structure elucidation with new strategies being constantly developed to improve the chances of crystallization. Generally, well-ordered epitopes possessing complementary surface and capable of producing stable inter-protein interactions generate a regular three-dimensional arrangement of protein molecules which eventually results in a crystal lattice. Metals, when used for crystallization, with their various coordination numbers and geometries, can generate such epitopes mediating protein oligomerization and/or establish crystal contacts. Some examples of metal-mediated oligomerization and crystallization together with our experience on metal-mediated crystallization of a putative rRNA methyltransferase from Sinorhizobium m

SUBMITTER: Hegde RP 

PROVIDER: S-EPMC5563152 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature