Estimation of flux ratios without uptake or release data: Application to serine and methionine metabolism.

ABSTRACT: Model-based metabolic flux analysis (MFA) using isotope-labeled substrates has provided great insight into intracellular metabolic activities across a host of organisms. One challenge with applying MFA in mammalian systems, however, is the need for absolute quantification of nutrient uptake, biomass composition, and byproduct release fluxes. Such measurements are often not feasible in complex culture systems or in vivo. One way to address this issue is to estimate flux ratios, the fractional contribution of a flux to a metabolite pool, which are independent of absolute measurements and yet informative for cellular metabolism. Prior work has focused on "local" estimation of a handful of flux ratios for specific metabolites and reactions. Here, we perform systematic, model-based estimation o

SUBMITTER: Nilsson R 

PROVIDER: S-EPMC5563485 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature