Profound changes in miRNA expression during cancer initiation by aflatoxin B₁ and their abrogation by the chemopreventive triterpenoid CDDO-Im.

ABSTRACT: Aflatoxin B₁ (AFB₁ ) is a potent human and animal hepatocarcinogen. To investigate the effects of aflatoxin on miRNA expression during the initiation phase of carcinogenesis, next-generation sequencing was used to analyze liver tissues from F344 rats exposed to 200 μg/kg per day AFB₁ for 4 week. A panel of miRNAs was identified that was upregulated with AFB₁ treatment compared to controls: rno-miR-434-3p, rno-miR-411-5p, rno-miR-221-3p, rno-miR-127-3p, rno-miR-205, rno-miR-429, rno-miR-34a-5p, rno-miR-181c-3p, rno-miR-200b-3p, and rno-miR-541-5p. Analysis of rat livers exposed to AFB₁ plus the chemopreventive triterpenoid CDDO-Im revealed a striking abrogation of this upregulation. These changes were validated by real-time PCR. We also

SUBMITTER: Livingstone MC 

PROVIDER: S-EPMC5563488 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature