Nucleotides upstream of the Kozak sequence strongly influence gene expression in the yeast S. cerevisiae.

ABSTRACT:

Background

In the yeast Saccharomyces cerevisiae, as in every eukaryotic organism, the mRNA 5^'-untranslated region (UTR) is important for translation initiation. However, the patterns and mechanisms that determine the efficiency with which ribozomes bind mRNA, the elongation of ribosomes through the 5^'-UTR, and the formation of a stable translation initiation complex are not clear. Genes that are highly expressed in S. cerevisiae seem to prefer a 5^'-UTR rich in adenine and poor in guanine, particularly in the Kozak sequence, which occupies roughly the first six nucleotides upstream of the START codon.

Results

We measured the fluorescence produced by 58 synthetic versions of the S. cerevisiae minimal CYC1 promoter (

SUBMITTER: Li J 

PROVIDER: S-EPMC5563945 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature