A pilot characterization of human lung NSCLC by protein pathway activation mapping.

ABSTRACT:

Background

An understanding of the activated protein signaling architecture in non-small-cell lung cancer (NSCLC) is of critical importance to the development of new therapeutic approaches and identification of predictive and prognostic biomarkers for patient stratification.

Methods

We used reverse-phase protein microarrays to map the activated protein signaling networks of 47 NSCLC tumors, 28 of which were node negative, which were subjected to tumor cellular enrichment using laser capture microdissection. The phosphorylation/cleavage levels of 111 key signaling proteins and total levels of 17 proteins were measured for broadscale signaling analysis.

Results

Pathway activation mapping of NSCLC revealed distinct subgroups composed of epidermal growth factor receptor (

SUBMITTER: Zupa A 

PROVIDER: S-EPMC5564308 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature