Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines.

ABSTRACT:

Purpose

The present study is to discover a new genes associated with drug resistance development in ovarian cancer.

Methods

We used microarray analysis to determine alterations in the level of expression of genes in cisplatin- (CisPt), doxorubicin- (Dox), topotecan- (Top), and paclitaxel- (Pac) resistant variants of W1 and A2780 ovarian cancer cell lines. Immunohistochemistry assay was used to determine protein expression in ovarian cancer patients.

Results

We observed alterations in the expression of 22 genes that were common to all three cell lines that were resistant to the same cytostatic drug. The level of expression of 13 genes was upregulated and that of nine genes was downregulated. In the CisPt-resistant cell line, we observed downregulated expression of ABCC

SUBMITTER: Januchowski R 

PROVIDER: S-EPMC5564819 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature